Date & Time
Search
Datum
{{range.dates[index].day}}
{{range.dates[index].date}}
Time
Mornings Noon Afternoons Evenings
  • from
  • to
  • o'clock
Topic
Event location
Event
Properties
{{item.name}}
{{item.name}}
Exhibition venue

(please choose the desired areas)

Lecture language
Format

Event database

The event database contains all event-related information for the World of Photonics Congress.

Back to the EventList

3D large-volume histological imaging of ex vivo tissue samples using inverted selective plane illumination microscopy (iSPIM)

JUN
24
2019
24. JUN 2019

Lecture Hall A1 European Conferences on Biomedical Optics (ECBO) > Preclinical and Clinical Optical Diagnostics > Novel Methods for Cell and Tissue Analysis

11:45-12:00 h | Hall A1 Room 6 "Charles Townes"

Subjects: Biophotonics and Medical Engineering

Chairman: Ton van Leeuwen (, Academisch UMC)

Type: Lecture

Speech: English

Better understanding of tissue structure at the microscopic scale in its 3D context is important for tissue structure-function study, disease diagnosis, and design and validation of new medical imaging modalities. Currently, tissue structures are usually studied by physically cutting tissue to 2D slices, then staining and imaging slices one by one. However, this process is destructive, time consuming, and limited to 2D shapes. Light sheet microscopy has been used for non-destructive pathology of large specimens in a traditional inverted configuration. However, imaging from underneath a sample that is pressed onto a glass slide or other sample container can distort the true 3D shape of the specimen, and can interfere with focusing location of the objective in relation to the true tissue surface in irregular or non-flat samples. Here, we explore the use of inverted selective plane illumination microscopy together with optical clearing to provide 3D high-content histological volume of different types of tissues, in various imaging mounting media, for the purposes of quantifying specific target cells or pathologies of interest in large tissue volumes for downstream research applications. It can track the natural shape of a specimen without any added material between objective lens and specimen other than the immersion medium. We have also demonstrated its capability of imaging samples to almost 2 mm deep. By dual-color imaging of D&E stained tissue and post-processing, 3D H&E-like images with nuclear resolution can be obtained, providing useful information for generating mathematical models of tissue structure-function, or for validation of low-resolution imaging systems.

Informations

Author,
Tulane Univ.

Bihe Hu
Bihe Hu

Location

Eingang
Nord-West
ICM
Eingang
Nord
Eingang
West
Atrium
Eingang
Nord-Ost
Eingang
Ost
Conference
Center Nord
Freigelände
C1
C2
C3
C4
C5
C6
B0
B1
B2
B3
B4
B5
B6
A1
A2
A3
A4
A5
A6

More Events