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Lecture Hall A1 European Conferences on Biomedical Optics (ECBO) > Preclinical and Clinical Optical Diagnostics > Novel Methods for Cell and Tissue Analysis
11:45-12:00 h | Hall A1 Room 6 "Charles Townes"
Subjects: Biophotonics and Medical Engineering
Chairman: Ton van Leeuwen (, Academisch UMC)
Better understanding of tissue structure at the microscopic scale in its 3D context is important for tissue structure-function study, disease diagnosis, and design and validation of new medical imaging modalities. Currently, tissue structures are usually studied by physically cutting tissue to 2D slices, then staining and imaging slices one by one. However, this process is destructive, time consuming, and limited to 2D shapes. Light sheet microscopy has been used for non-destructive pathology of large specimens in a traditional inverted configuration. However, imaging from underneath a sample that is pressed onto a glass slide or other sample container can distort the true 3D shape of the specimen, and can interfere with focusing location of the objective in relation to the true tissue surface in irregular or non-flat samples. Here, we explore the use of inverted selective plane illumination microscopy together with optical clearing to provide 3D high-content histological volume of different types of tissues, in various imaging mounting media, for the purposes of quantifying specific target cells or pathologies of interest in large tissue volumes for downstream research applications. It can track the natural shape of a specimen without any added material between objective lens and specimen other than the immersion medium. We have also demonstrated its capability of imaging samples to almost 2 mm deep. By dual-color imaging of D&E stained tissue and post-processing, 3D H&E-like images with nuclear resolution can be obtained, providing useful information for generating mathematical models of tissue structure-function, or for validation of low-resolution imaging systems.