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Lecture Hall A1 SPIE Optical Metrology > Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV > Advanced Diagnostics by Speckle Techniques
12:30-12:50 h | Hall A1 Room 8 "Gustav Hertz"
Subjects: Optical Measurement Systems / Optical Metrology
Chairman: Aydogan Ozcan (, Univ. of California, Los Angeles)
A novel method for sensing low abundant lactose in small sample volumes is proposed. It is based on a pyro-electrodynamic jet (p-jet) system able to concentrate the lactose molecules onto a solid amine support for easy and rapid detection through a fluorescence measurement. The p-jet produces droplets with sub-picoliter volumes accumulated onto a micrometer area of the support in order to reduce the diffusion limits typically occurring in standard well-based assays. A highly reproducible linear response for lactose was obtained between 2 pM and 10 pM/uL. The great advantage of the technique is the ability to concentrate the molecules directly onto the solid support ready to for the readout measurement by a standard fluorescence scanner. No time-consuming and expensive sample treatments are needed. The proposed method is rapid, suitable for repeated use providing a built-in quality assurance.